Isolation and screening of amylase producing Bacillus species from soil Aneela

Isolation and screening of amylase producing Bacillus species from soil Aneela

Abstract

Amylases are one of the most important  industrial enzymes, which hydrolyze starch molecules to fine products  such as dextrins, maltose  etc. In recent years, interest in the microbial production  of  enzyme  has increased dramatically due to its wide spread use in baking, food, textile, and detergent industry. Amylase  production  by  bacteria esp. by Bacillus  species  is  of  great  importance  nowadays. The  purpose  of  current  investigation  is  to  isolate  amylase  producing  Bacillus  species  from  soil. Soil samples were collected from different areas of Karachi,  Pakistan. Samples were analyzed for bacteriological study.  89 different  bacterial  species  were isolated  from different soil samples. Morphological and biochemical  identification  revealed  39 Bacillus species. These  species  were  screened  for  amylase  production.  Five  species  were selected as  highest  enzyme  producers  in  starch  hydrolysis  test.  Among  the  five,  AS-2 was  found  to  be  the  highest  enzyme  producing  specie  in enzyme  assay.  The  enzyme  activity  was  found  to  be  3179.62  IU/ml/min.  Such specie   could  be  used  as the  auspicious source of amylase in  industry.   

No. of Pages
151-164
Volume
3
Author's Details
Aneela Rehman and Asma Saeed*
Research Field
Microbiology (Enzymology)