Diversity, Community Composition and Abundance of Anammox Bacteria in Sediments of the North Marginal Seas of China

Diversity, Community Composition and Abundance of Anammox Bacteria in Sediments of the North Marginal Seas of China

Abstract

    L           i    of large amounts of nitrogen in the global marine nitrogen cycle. In the present study, the diversity, community composition, and abundance of anammox bacteria from the sediments of four diverse regions in the north marginal seas in China were determined via clone library construction and a quantitative PCR analysis. The clone libraries retrieved by the 16S rRNA gene and Hzo gene markers indicated that “Candidatus Scalindua” was the predominant group throughout the sites examined. The 16S rRNA gene clone libraries revealed exceptional diversity by identifying two potential novel anammox clades, as evidenced by the high sequence similarities between these two clades and known anammox genera, and their unique phylogenetic positions with high bootstrap values. However, their potential roles in the anammox reaction need to be validated. Six novel members of Planctomycetes, divergent from the known genera of anammox bacteria, were also detected. A phylogenetic analysis by Hzo protein sequences revealed the existence of two known genera, i.e., “Candidatus Jettenia” and “Candidatus Anammoxoglobus”, which are rarely captured from marine sediments. Among all ecological parameters investigated, the              L   L  temperature. The abundance of the anammox bacterial 16S rRNA gene from the sites examined ranged between 3.95×105 and 9.21×105 copies g–1 wet sediment and positively correlated with the median size of the sediment sample. 

No. of Pages
111-120
Volume
31
Author's Details
Ahmed ShehzAd1†, Jiwen Liu1†, min Yu1, ShAkeeLA QiSmAt1, JingLi Liu1, and XiAo-huA zhAng1,2*
Research Field
Microbiology