Seroprevalence and comparison of three different screening tests for detection of human immunodeficiency virus infection in Peshawar, Pakistan

Seroprevalence and comparison of three different screening tests for detection of human immunodeficiency virus infection in Peshawar, Pakistan

Abstract

Human immunodeficiency virus (HIV) concedes as a significant public health issue in the world from decades, which is
growing speedily in number. Although, there seems to be insufficient data regarding HIV infection seroprevalence in general
population of Peshawar, Pakistan. The objectives of the current study was to determine the seroprevalence of HIV infection in
the general population of Peshawar, Pakistan and to compare the performance of three different screening methods for
detection of HIV infection. A total of 5370 blood samples were screened for anti-HIV antibodies and HIV-RNA by using
immunochromatographic test (ICT), enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase
chain reaction (RT-PCR) at Antiretroviral Therapy (ART) centre in Hayatabad Medical Complex (HMC), Peshawar. Out of
5370 blood samples, 756 (14.07%) were positive by ICT, 639 (11.89%) by ELISA and 606 (11.28%) by RT-PCR. The active HIV
infection was high in males, i.e. 468 (13.61%) than in females 138 (7.14%). It was observed that infection was highest in the age
group of 21–30 years that was about 237 (16.80%), whereas no sign of infection was observed for the age group of 01-10 years.
However, the married population had an HIV seroprevalence of 423 (13.83%) while singles had 183 (7.91%) respectively.
ELISA should be preferred for anti-HIV antibodies detection over ICT and RT-PCR should be preferred over ELISA for HIV
RNA detection. Moreover, the provision of treatment and screening facilities against this virus should be assured in medical
care units of rural areas to prevent this infection.

 

No. of Pages
169-174
Volume
15 (3)
Author's Details
Saba, Iqbal Hussain, Nasir Ali, Muhammad Junaid, Rahim Dost Khan, Safia Hameed, Muhammad Waqas, Jawaria Shah
Research Field
Microbiology