Cytological characterization of anther culture derived plants from the interspecific crosses between Oryza sativa x Oryza australinesis and Oryza sativa x Oryza brachyantha

Cytological characterization of anther culture derived plants from the interspecific crosses between Oryza sativa x Oryza australinesis and Oryza sativa x Oryza brachyantha

Abstract

Anther culture is being used in cereal crop improvement both as a source of haploids and for inducing
the new genetic variability. We studied the possibilities of producing the aneuploids in rice by anther
culture. Anthers for callus induction were plated on semi solid media. Callus formation was observed
20 days after plating. The anthers became necrotic prior to callus formation. Tapei 309 exhibited the
highest callus induction efficiency (7.8%) followed by IR31917-45-3-2 (7.7%). IR56 produced (1.45%)
callus. The F1 hybrids (IR31917-45-3-2 x Oryza australiensis) and IR56 x Oryza brachyantha responded
poorly for callus induction (0.033%) and (0.030%) respectively. The 879 calli of IR31917-45-3-2
regenerated into six (0.68%) green plants and 79 (8.9%) albinos. All the green plants were haploids. No
green plant was produced from the 112 calli of IR56. Similarly, the calli from IR56 x O. brachyantha did
not show any plant regeneration. The calli from the F1 hybrid (IR31917-45-3-2 x O. australiensis) yielded
six (20.6%) green and two (6.89%) albino plants. Three of the six plants did not grow after regeneration,
while the remaining three plants were used for cytological studies. One plant was aneuploid with 27
chromosomes. Genomic in situ hybridization (GISH) using biotin labeled genomic DNA unequivocally
detected 14 chromosomes of O. australiensis and 13 chromosomes from IR31917-45-3-2 in this
aneuploid. Similarly, in the other two plants with 24 chromosomes each, the 12 chromosomes of O.
australiensis could be discriminated from the 12 chromosomes from IR56.
 

No. of Pages
05 (3269-3273)
Volume
Vol. 10(17), pp. 3269-3273, 25 April, 2011
Author's Details
Fida M Abbasi, Kehkashan Akbar, Mujaddad Ur Rehman, Muhammad Tariq Khan, Sajjad Iqbal, Anees Fatima, Noshine, Hamid Ali and Muniba Fida Abbasi
Research Field
Plant Biotechnology