Calcium malate overproduction by Penicillium viticola 152 using the medium containing corn steep liquor

Calcium malate overproduction by Penicillium viticola 152 using the medium containing corn steep liquor

Abstract

Abstract In this study, after screening of eight fungal strains
for their ability to produce calcium malate, it was found that
Penicillium viticola 152 isolated from marine algae among
them could produce the highest titer of calcium malate. At the
same time, it was found that corn steep liquor (CSL) could
stimulate calcium malate production and 0.5%(v/v) CSL was
the most suitable for calcium malate production. Under the
optimal conditions, a titer of calcium malate in the supernatant
was 132 g/l at flask level. During a 10-l fermentation, a titer of
168 g/l, a yield of 1.28 g/g of glucose, and a productivity of
1.75 g/l/h were reached within 96 h of the fermentation, and
93.4 % of the sugar was used for calcium malate production
and cell growth, demonstrating that the titer, yield, and productivity
of calcium malate by this fungal strain were very
high and the fermentation period was very short. After analysis
of the partially purified product with high-performance
liquid chromatography, it was found that the main product was
calcium malate. The results demonstrated that P. viticola 152
obtained in this study was the most suitable for developing a
novel one-step fermentation process for calcium malate production
from glucose on a large scale.

No. of Pages
1539-46
Volume
98
Author's Details
Ibrar Khan & Kiran Nazir & Zhi-Peng Wang & Guang-Lei Liu & Zhen-Ming Chi
Research Field
Microbiology