High-level production of calcium malate from glucose by Penicillium sclerotiorum K302

High-level production of calcium malate from glucose by Penicillium sclerotiorum K302

Abstract

In this study, after screening of 9 fungal strains for their ability to produce calcium malate, it was found
that Penicillium sclerotiorum K302 among them could produce high-level of calcium malate. Under the
optimal conditions, the titer of calcium malate in the supernatant was 88.6 g/l at flask level. During
10-l fermentation, the titer of 92.0 g/l, the yield of 0.88 g/g of glucose and the productivity of 1.23 g/l/h
were reached within 72 h of the fermentation, demonstrating that the titer, yield and productivity of calcium
malate by this strain were very high and the fermentation period was very short. After analysis of
the partially purified product with HPLC, it was found that the main product was calcium malate. The
results showed that P. sclerotiorum K302 obtained in this study was suitable for developing a novel
one-step fermentation process for calcium malate production from glucose on large scale.

No. of Pages
674–677
Volume
143
Author's Details
Zhi-Peng Wang, Guang-Yuan Wang, Ibrar Khan, Zhen-Ming Chi
Research Field
Microbiology