Bacteriological Analysis of Siran River System for Fecal Contamination and Metallo-β-Lactamase blaNDM-1 Gene

Bacteriological Analysis of Siran River System for Fecal Contamination and Metallo-β-Lactamase blaNDM-1 Gene

Abstract

Abstract.- Musa ka Musalla glacier is situated in the Allai, Manshera District, Khyber Pakhtunkhwa, Pakistan
(34.713678, 73.360806). There were 20 different water samples sites moving downstream from the glacier, which is
the source of river Siran, during winter, summer and monsoon seasons taken for the analysis of water quality
destruction in sense of total viable count, total coliform count, fecal coliform count, fecal E.coli count, which are
indicator organisms of fecal contamination, as well as the prevalence and distribution of MBL blaNDM-1 producing
Gram negative bacteria. Total viable counts were found in the range of 3×10² to 40×10² CFU/mL in winter, 5×10² to
45×10² CFU/mL in summer and 2×10² to 40×10² CFU/mL in monsoon. The range of MPN 100 mL-1 for total coliform
was found 11–2400, 150–2400 and 80–2400, for fecal coliform, it was 3–700, 2–900, 2–800 and for fecal E.coli, it
was 1–300, 2–450 and 1–400 in winter, summer and monsoon, respectively. While, out of total 20 samples from
different sites 07 were positive to MBL producing Gram negative bacteria and among 07 Only 03 isolates including
Dadar (U-4), Shinkiari (M-10) and Khaki (M-13) were blaNDM-1 positive. The overall objective of this work is to
examine the incidence of these indicator organisms, coliforms, fecal coliforms, fecal E.coli and as well as blaNDM-1
gene occurrence in isolates in runoff of the Musa ka Musalla glacier, Khyber Pakhtunkhwa, Pakistan.

No. of Pages
707-716
Volume
46
Author's Details
Ibrar Khan, Muhammad Awais, Mudassar Shah, Tawaf Ali Shah, Shehzad Ahmed, Muhammad Junaid and Safia Ahmed
Research Field
Microbiology