Biological evaluation and preliminary screening of endophytes of Taxus fauna for taxol production

Biological evaluation and preliminary screening of endophytes of Taxus fauna for taxol production

Abstract

Endophytic fungi of Taxus species, being considered as economically most promising source for taxol
production, prompted the current study for screening of endophytic fungi associated with Taxus fuana of
Pakistan. Two endophytic fungi Epicoccum sp.NFW1 and MucorhemalisNFW6 were explored for their biological
activities and taxol production by utilizing bioassays, TLC, RP-HPLC and LC-ESI-MS analyses. Crude extract of
NFW1 showed significant antimicrobial activity with zone of inhibition ranging from 11.8 - 23.1 mm against
different test strains. It also expressed cytotoxicity in brine shrimp lethality assay with an IC50 value 10μg/mL.
NFW6 expressed moderate antimicrobial activity and high antioxidant potential (89.2%) with IC50 51.2 μg/mL in
DPPH assay. RP-HPLC confirmed the presence of taxol in NFW1 at a concentration 280.7 μg/g of dry mycelial
mass. It was further validated by LC-ESI-MS analysis. These findings reveal that Epicoccum sp.NFW1 should be
studied for the isolation and characterization of taxol and related metabolites.

No. of Pages
38-46
Volume
7
Author's Details
Muniba Jadoon, Nighat Fatima, Ibrar Khan, Bushra Mirza, Ihsan-Ul-Haq, Muneer Ahmed Qazi, Safia Ahmed
Research Field
Microbiology